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KMID : 0377519950200010001
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Chung-Ang Journal of Medicine
1995 Volume.20 No. 1 p.1 ~ p.14
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Cultures of Rabbit Chondrocytes Embedded in Fibrin Glue
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하영준
장의찬/정상인/강수용/유재형/이은우
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Abstract
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Chondrocytes isolated from rabbit articular cartilage were cultured in vitro within fibrin glue carrier (Beriplast-p) for 2 weeks. Histochemical and electronmicroscopic approaches were used to study chondrocyte behavior and phenotypic expression.
Under these culture condition, chondrocytes anssumed a rounded morphology, accumulated metachromatic matrix, and took on the cytological characteristics of in vivo cartilage cells. No signs of cytotoxic effects by fibrin glue were observed.
Most of the metachromatic synthesized matrix were deposited within the fibrin glue, and areas of matrix increased with time during first 2 weeks.
It was clear that this cell-culture system, using periplant, provides chondrocytes with a suitable environment in which accumulate synthesized matrix macromolecules and preserve their diffentiated phenotype in vitro.
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